diff --git a/tools/interop_matrix/client_matrix.py b/tools/interop_matrix/client_matrix.py
index 73dec5d50ee..0568b179788 100644
--- a/tools/interop_matrix/client_matrix.py
+++ b/tools/interop_matrix/client_matrix.py
@@ -206,6 +206,7 @@ LANG_RELEASE_MATRIX = {
             ('v1.46.0', ReleaseInfo(runtimes=['go1.16'])),
             ('v1.47.0', ReleaseInfo(runtimes=['go1.16'])),
             ('v1.48.0', ReleaseInfo(runtimes=['go1.16'])),
+            ('v1.49.0', ReleaseInfo(runtimes=['go1.16'])),
         ]),
     'java':
         OrderedDict([