diff --git a/tools/interop_matrix/client_matrix.py b/tools/interop_matrix/client_matrix.py
index e5aad848f6c..2b5c1c9c112 100644
--- a/tools/interop_matrix/client_matrix.py
+++ b/tools/interop_matrix/client_matrix.py
@@ -133,6 +133,7 @@ LANG_RELEASE_MATRIX = {
             ("v1.61.0", ReleaseInfo()),
             ("v1.62.0", ReleaseInfo()),
             ("v1.63.0", ReleaseInfo()),
+            ("v1.64.0", ReleaseInfo()),
         ]
     ),
     "go": OrderedDict(
@@ -789,6 +790,12 @@ LANG_RELEASE_MATRIX = {
                     runtimes=["python"], testcases_file="python__master"
                 ),
             ),
+            (
+                "v1.64.0",
+                ReleaseInfo(
+                    runtimes=["python"], testcases_file="python__master"
+                ),
+            ),
         ]
     ),
     "node": OrderedDict(
@@ -886,6 +893,7 @@ LANG_RELEASE_MATRIX = {
             ("v1.61.0", ReleaseInfo()),
             ("v1.62.0", ReleaseInfo()),
             ("v1.63.0", ReleaseInfo()),
+            ("v1.64.0", ReleaseInfo()),
         ]
     ),
     "php": OrderedDict(
@@ -947,6 +955,7 @@ LANG_RELEASE_MATRIX = {
             ("v1.61.0", ReleaseInfo()),
             ("v1.62.0", ReleaseInfo()),
             ("v1.63.0", ReleaseInfo()),
+            ("v1.64.0", ReleaseInfo()),
         ]
     ),
     "csharp": OrderedDict(