diff --git a/test/distrib/csharp/run_distrib_test_dotnetcli.sh b/test/distrib/csharp/run_distrib_test_dotnetcli.sh
index 97bef26d399..69257edde43 100755
--- a/test/distrib/csharp/run_distrib_test_dotnetcli.sh
+++ b/test/distrib/csharp/run_distrib_test_dotnetcli.sh
@@ -68,9 +68,14 @@ if [ "${SKIP_NET50_DISTRIBTEST}" != "1" ]
 then
   dotnet publish -f net5.0 DistribTestDotNet.csproj
 
+  dotnet publish -r linux-x64 -f net5.0 DistribTestDotNet.csproj -p:PublishSingleFile=true --self-contained true --output net5_singlefile_publish
+
   # .NET Core target after dotnet build
   dotnet exec bin/Debug/net5.0/DistribTestDotNet.dll
 
   # .NET Core target after dotnet publish
   dotnet exec bin/Debug/net5.0/publish/DistribTestDotNet.dll
+
+  # binary generated by the single file publish
+  ./net5_singlefile_publish/DistribTestDotNet
 fi